Concentration calculation from UV Vis absorbance
STEP1: Open the absorbance graph of the solution, which is obtained from the UV Vis spectroscopy.
STEP2: Now zoom on the peak for which you want to calculate the concentration and note down the Absorbance value.
STEP3: Now enter the measured absorbance value (eg. 0.84) into the “Absorbance of Solution” column of the calculator; also enter the value of “Molar Absorptivity” (eg. 19400) of that material from the literature survey and “Solution Cell Length” (eg. 1 cm) in the next columns. You should get the calculated results of the band gap in the “Calculated Result” field.
NOTE: For accuracy you can make a solution for known concentration and enter the values of “Absorbance of Solution”, “Solution Cell Length” and “Known Concentration of Solution” in the given columns. You should get the value of “Molar Absorptivity” from the calculator.
Then enter the value of “Molar Absorptivity” in the above calculator and find the exact concentration of solution from the above calculator.
Theory Behind Calculations:
Calculations are based on the Beer’s Lambert law given by:
A = ε l c
Where A = Absorbance of solution at a particular wavelength; ε = Molar Absorptivity; l = Length of Solution Cell; and c = Concentration of Solution (mol/dm3)