Synthesis of Silver Nanoparticles by Leaf Extract
CHECK LIST: Fresh leaves of Bottle Brush or F. Panda, Silver Nitrate, Deionized Water, Beaker, Buret, Magnetic Stirrer with Temperature option, FIlter Paper
STEP1: Take fresh leaves of Bottle Brush or F. Panda. Wash properly and cut them into small pieces.
STEP2: Take Leaf pieces (25g) and mix with Deionized Water (100ml)
STEP3: Now boil the Leaves at around 80-90ºC for 5-10 minutes
STEP4: When the water turns GREEN, filter it out and use it as reducing and capping agent for Nanoparticles synthesis
STEP5: Take Silver Nitrate (10mg) in deionized water (50ml), cover conical flask with Aluminium foil to avoid photo-degradation; and set temperature 60-70ºC
STEP6: Add leaf extract drop wise until the color of Silver Nitrate solution turns light Yellow.
RESULTS: The light yellow color indicated the formation of Silver Nanoparticles in water. UV-Vis spectroscopy absorption peak would be obtained around 420nm.
FACTORS AFFECTING THE SYNTHESIS:
- Species of Plant/Tree: Different types of Plants/Trees are having different types of chemical composition and concentration of specific chemical in its leaves. So different species of Plants/Trees can give the different particle size, size distribution. Even morphology can also be changed by using leaf extract of different plants/trees.
- Concentration of Silver Nitrate: Silver Nitrate is used as the precursor in this synthesis. Higher the concentration of Silver Nitrate, more the chances of agglomeration; further leads to bulky size particles.
- Concentration of Leaves in Water: Concentration of leaves in water is very important for Nanoparticles synthesis. Higher concentration of leaves in water, leads to higher concentration of reducing and capping agent in water.
- Temperature: The temperature is another important factor in the synthesis of Nanoparticles, change in temperature leads to change in the particles size; also higher temperature leads to faster chemical Kinetics.
NOTE: All the experiments should be done under the guidance of lab Incharge; and proper lab safety instructions.