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Leaf Extract Based Nanoparticles Green Synthesis via Biological Method

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Leaf Extract Based Nanoparticles Green Synthesis via Biological Method - InstaNANO. https://instanano.com/all/nanomaterial-synthesis/leaf-extract-synthesis/ (accessed May 6th, 2025).

Nanoparticles can be easily synthesized by using various leaf extracts as reducing and capping agent with various precursors.

  1. CHECK LIST
    Fresh leaves of desire tree/plant, Deionized Water, RB Flask, Dropper, Magnetic Stirrer with Temperature option, FIlter Paper

  2. STEP 1.
    Take fresh leaves of your choice plant. Wash properly and cut them into small pieces

  3. STEP 2.
    Take 25g leaves pieces in 100ml of deionized water and boil the leaves around 80-90ºC for 5-10 minutes.

  4. STEP 3.
    When the water turns GREEN, filter it out and use it as reducing and capping agent for Nanoparticles synthesis.

  5. RESULTS
    The color of used precursor solution will change depending on the type of precursor; indicates the formation of that precursor Nanoparticles.

  6. Factors Affecting Synthesis
    Species of Plant/Tree: Different types of Plants/Trees are having different types of chemical composition and concentration of specific chemical in its leaves. So different species of Plants/Trees can give the different particle size, size distribution. Even morphology can also be changed by using leaf extract of different plants/trees.
    Concentration of Copper Nitrate: Nickel Chloride is used as the precursor in this synthesis. Higher the concentration of Nickel Chloride, more the chances of agglomeration; further leads to bulky size particles.
    Concentration of Leaves in Water: Concentration of leaves in water is very important for Nanoparticles synthesis. Higher concentration of leaves in water, leads to higher concentration of reducing and capping agent in water.
    Temperature: The temperature is another important factor in the synthesis of Nanoparticles, change in temperature leads to change in the particles size; also higher temperature leads to faster chemical Kinetics.

  7. NOTE: All the experiments should be done under the guidance of lab Incharge; and proper lab safety instructions.

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