Silver Nanoparticles Green Synthesis by using Silver Nitrate and American Holly Leaves via Biological Method

1. CHECK LIST

   Silver Nitrate, Fresh leaves of American Holly (Ilex opaca), Deionized Water, RB Flask, Dropper, Magnetic Stirrer with Temperature option, FIlter Paper
   

2. STEP 1

   Take fresh leaves of American Holly. Wash properly, remove the midrib, and cut them into small pieces.
   

3. STEP 2

   Take 5g leaves pieces in 50ml of deionized water and boil the leaves around 80ºC for 20 minutes.
   

4. STEP 3

   When the water turns green, stop the temperature and take out round bottom flask from the hot plate.
   

5. STEP 4

   Filter out this green color extract using a filter paper. This extract will be used as reducing and capping agent for Nanoparticles synthesis.
   

6. STEP 5

   Take 100mg Silver Nitrate salt in 100ml deionized water; and set the temperature 60ºC.

   (NOTE: Silver Nitrate degrades in Silver Oxide (Black color) in light, so wrap flask with aluminium foil to avoide this.)

   

7. STEP 6

   Add 5ml leaf extract drop wise slowly (prepared in step 4) into Silver Nitrate solution, until the color changes (around 20-30 minutes) to light YELLOW.
   

8. RESULTS

   The light Yellow color indicated the formation of Silver Nanoparticles in water. UV-Vis spectroscopy absorption peak would be obtained around 420nm.
   

9. Note

   All the experiments should be done under the guidance of lab Incharge; and proper lab safety instructions.